Chromosomal DNA deletion confers phage resistance to Pseudomonas aeruginosa

نویسندگان

  • Shuai Le
  • Xinyue Yao
  • Shuguang Lu
  • Yinling Tan
  • Xiancai Rao
  • Ming Li
  • Xiaolin Jin
  • Jing Wang
  • Yan Zhao
  • Nicholas C. Wu
  • Renate Lux
  • Xuesong He
  • Wenyuan Shi
  • Fuquan Hu
چکیده

Bacteria develop a broad range of phage resistance mechanisms, such as prevention of phage adsorption and CRISPR/Cas system, to survive phage predation. In this study, Pseudomonas aeruginosa PA1 strain was infected with lytic phage PaP1, and phage-resistant mutants were selected. A high percentage (~30%) of these mutants displayed red pigmentation phenotype (Red mutant). Through comparative genomic analysis, one Red mutant PA1r was found to have a 219.6 kb genomic fragment deletion, which contains two key genes hmgA and galU related to the observed phenotypes. Deletion of hmgA resulted in the accumulation of a red compound homogentisic acid; while A galU mutant is devoid of O-antigen, which is required for phage adsorption. Intriguingly, while the loss of galU conferred phage resistance, it significantly attenuated PA1r in a mouse infection experiment. Our study revealed a novel phage resistance mechanism via chromosomal DNA deletion in P. aeruginosa.

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عنوان ژورنال:

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2014